Biology - Biotechnology - Principles and Processes
Practise 17 NEST (NISER) previous year questions on Biotechnology - Principles and Processes, compiled from papers spanning 2011 to 2026. Each question is pulled directly from the actual exam paper it appeared in, so you're practising in the exact style and difficulty NEST (NISER) tests.
Biotechnology - Principles and Processes has been asked in 11 of the 16 years of NEST (NISER) papers analysed (2011–2026), with 17 questions in all. 18% of them were easy, 71% moderate and 12% tough. Most of its questions came from 2024, with 3 questions that year. In the last three years (2024–2026) it had 4 questions across 4 papers, about 1 per paper.
The chapter's 3 topics are not weighted equally: Tools of Recombinant DNA Technology (14 questions, 82%), Principles of Biotechnology (2 questions, 12%), and Conceptual Development of Genetic Engineering (1 question, 6%) have been asked the most. Tools of Recombinant DNA Technology has also led over the last five years, with 8 questions. Tools of Recombinant DNA Technology has produced the most tough questions (2).
- 1A researcher wants to insert a linear DNA fragment into a plasmid DNA. The linear DNA fragment has two MfeI restriction sites at its ends. T2026Tools of Recombinant DNA Technology
- 2In the vector map, GFP denotes the green fluorescent protein, is a tetracycline resistance gene and is an ampicillin resistance gene. A DNA 2024 (Session 1)Tools of Recombinant DNA Technology
- 3A researcher was trying to amplify a DNA fragment in vitro using a polymerase chain reaction (PCR). All the required components for the reac2024 (Session 2)Tools of Recombinant DNA Technology
- 4In the plasmid vector map denotes tetracycline resistance gene and denotes ampicillin resistance gene. A SalI restriction enzyme-digested DN2024 (Session 2)Tools of Recombinant DNA Technology
- 5Restriction enzymes recognize certain sequences within the DNA and cleave them. If a DNA fragment is cleaved with BamHI restriction enzyme, 2023 (Session 2)Tools of Recombinant DNA Technology
- 6An experimenter ligated cDNA of gene into the multiple cloning site (MCS) of a vector whose map is shown below. The ligated vector was trans2022 (Session 1)Tools of Recombinant DNA Technology
- 7Primers and have binding sites on the template DNA as depicted in the figure below. In a polymerase chain reaction (PCR) using all four prim2022 (Session 2)Tools of Recombinant DNA Technology
- 8Schematic shows restriction enzyme site mapping on a DNA fragment containing gene that codes for protein . Schematic shows a plasmid-based v2022 (Session 2)Tools of Recombinant DNA Technology
- 9In four separate experiments (Table), a vector DNA pBxyz ( 6000 bp ) and a DNA-X (1500 bp) fragment was digested with the indicated restrict2021 (Session 2)Tools of Recombinant DNA Technology
- 10The 2020 Nobel prize in chemistry was awarded for the CRISPR/Cas9 system. This concept was derived from2021 (Session 2)Conceptual Development of Genetic Engineering
- 11A pure culture of Escherichia coli was streaked on a plate to get single isolated colonies. The plasmid DNA from two such colonies was indiv2020 (Session 1)Tools of Recombinant DNA Technology
- 12A polymerase chain reaction (PCR) for amplification of DNA fragments involves multiple cycles of thermal denaturation, annealing and extensi2020 (Session 2)Tools of Recombinant DNA Technology
- 13A purified linear double-stranded DNA fragment was independently subjected to five treatments using different restriction enzymes (RE) and t2019 (Session 2)Tools of Recombinant DNA Technology
- 14In a cloning experiment using E. coli, a DNA fragment was inserted in an EcoRI restriction site of a plasmid vector that contained the and g2015Tools of Recombinant DNA Technology
- 15Induced pluripotent stem cells are formed by2014Principles of Biotechnology
- 16E. coli is a bacterium that is routinely used in cloning experiments and can be grown on artificially synthesized media in the laboratory. O2012Tools of Recombinant DNA Technology
- 17The animal cloning technique adopted in the case of 'Dolly' the sheep involved the2011Principles of Biotechnology
Frequently Asked Questions
1.How many questions are available for Biotechnology - Principles and Processes?
This chapter includes 17 questions compiled from NEST (NISER) papers spanning 2011 to 2026.
2.Are these questions only from NEST (NISER), or from other exams too?
These are NEST (NISER) questions specifically for the Biotechnology - Principles and Processes chapter. Questions from other exams like JEE Main or NTA Abhyas (JEE Main) are organised separately under their respective exam sections.
3.Is Biotechnology - Principles and Processes an important chapter for NEST (NISER) Biology?
Biotechnology - Principles and Processes has appeared in 11 of the 16 years of NEST (NISER) papers covered here, with 17 questions across 14 papers - about one per paper it appears in.
4.What is the difficulty level of Biotechnology - Principles and Processes questions in NEST (NISER)?
Of the 17 questions analysed for this chapter, 18% are easy, 71% moderate and 12% tough. 2024 had the most, with 3 questions.
5.Which topics in Biotechnology - Principles and Processes should I focus on?
These topics have carried the most weightage in Biotechnology - Principles and Processes:
- Tools of Recombinant DNA Technology - 14 questions (82%)
- Principles of Biotechnology - 2 questions (12%)
- Conceptual Development of Genetic Engineering - 1 question (6%)
- Tools of Recombinant DNA Technology has produced the most tough questions (2).
6.Is Biotechnology - Principles and Processes's weightage in NEST (NISER) going up or down?
Across all 14 papers analysed, Biotechnology - Principles and Processes has averaged about 1.2 questions per paper. In the last three years (2024–2026), that average has fallen, at about 1.0 questions per paper. Use the year-wise numbers on this page for the exact trend before you decide how much time to give it.