Schematic $\mathbf{P}$ shows restriction enzyme site mapping on a DNA fragment containing gene $\boldsymbol{w}$ that codes for protein $\mathbf{W}$. Schematic $\mathbf{Q}$ shows a plasmid-based vector map and restriction enzyme recognition site sequences in the multiple cloning site (MCS). The oblique lines in the sequences next to each restriction enzyme name show the cleavage sites of the respective restriction enzymes. If the goal of an experimenter is to clone the gene $\boldsymbol{w}$ into the vector to express the protein $\mathbf{W}$, the correct restriction enzyme pair(s) from the options below that can be used to digest the DNA fragment as well as the vector is/are:

• Correct answer
  A

  Hind III and $A p a \mathrm{I}$
• B

  Hind III and EcoRV
• Correct answer
  C

  $E c o \mathrm{RV}$ and $A p a \mathrm{I}$
• D

  BamHI and EcoRV

Hence, the correct answer is options: 'A', 'C'.