In the vector map, GFP denotes the green fluorescent protein, $T e t^R$ is a tetracycline resistance gene and $A m p^R$ is an ampicillin resistance gene. A DNA fragment containing the gene of interest was generated using SalI digestion. This fragment was ligated with the SalI digested vector. Assume complete digestion by SalI. E. coli was transformed with the ligation product and transformants were plated on media. The recombinants having the gene of interest will grow on media containing ______.

• A

  ampicillin and are GFP positive
• Correct answer
  B

  tetracycline and are GFP negative
• C

  ampicillin and are GFP negative
• D

  tetracycline and are GFP positive

Hence, the correct answer is option: 'B'.