Biology - Biomolecules (B)
Practise 29 NEST (NISER) previous year questions on Biomolecules (B), compiled from papers spanning 2011 to 2024. Each question is pulled directly from the actual exam paper it appeared in, so you're practising in the exact style and difficulty NEST (NISER) tests.
Biomolecules (B) has been asked in 11 of the 16 years of NEST (NISER) papers analysed (2011–2026), with 29 questions in all. 7% of them were easy, 79% moderate and 14% tough. Most of its questions came from 2024, with 7 questions that year. In the last three years (2024–2026) it had 7 questions across 4 papers, about 1.8 per paper.
The chapter's 4 topics are not weighted equally: Enzymes (15 questions, 52%), Protein (8 questions, 28%), and Nucleic Acid (4 questions, 14%) have been asked the most. Enzymes has also led over the last five years, with 5 questions. Protein has produced the most tough questions (3).
- 1Katalin Karikó and Drew Weissman were awarded the Nobel Prize in Physiology or Medicine for the year 2023 for their observation that pseudo2024 (Session 1)Nucleic Acid
- 2Analyze the images and deduce the correct structural description of the molecules.2024 (Session 1)Protein
- 3The graph depicts energy diagram (potential energy vs progress of reaction) of an uncatalyzed (black solid line) and enzyme-catalysed (red s2024 (Session 1)Enzymes
- 4Identify the non-reducing disaccharide:2024 (Session 1)Carbohydrates
- 5The amino acid sequence of an unknown peptide can be determined using a series of proteolytic digestions. An eight-mer peptide contains 1 al2024 (Session 2)Protein
- 6Depicted in the figure are three possibilities of substrate (white triangle) conversion to the product (gray triangle) by an enzyme (blue) i2024 (Session 2)Enzymes
- 7Camels have three different sub-classes of and , of which IgG2 and IgG3 are structurally different from the human IgG. A researcher purified2024 (Session 2)Protein
- 8A biochemist wants to purify a protein X (molecular weight and ) from a solution containing proteins X and Y (molecular weight and using ion2023 (Session 1)Enzymes
- 9The table below presents the kinetic data obtained for an enzyme in the absence and presence of two different inhibitors P and Q , each at a2023 (Session 2)Enzymes
- 10A student working in a lab had three tubes with the following preparations: (i) Freshly extracted nucleic acids dissolved in a buffer. (ii) 2022 (Session 1)Nucleic Acid
- 11A biochemical reaction is catalysed by an enzyme . An experimenter measured the amount of product formed in the reaction either in an uncata2022 (Session 1)Enzymes
- 12The quantities and were measured independently for an enzymatic reaction using enzymes E1, E2, E3 and E4 isolated from four different organi2021 (Session 1)Enzymes
- 13A protein with isoelectric point 6.5 was isolated. It was prepared in three different buffers and with and 8.5 , respectively. With respect 2021 (Session 2)Protein
- 14A researcher determined the initial velocity of an enzyme in three different reactions (corresponding to lines and in the graph) with increa2021 (Session 2)Enzymes
- 15Three given polypeptides and R , of same size and same , have a single aspartic acid in each of them. In polypeptide , the aspartic acid res2020 (Session 1)Protein
- 16Monomeric subunits of proteins can sometimes assemble into higher order assemblies. Two such related human proteins and assemble into hexame2020 (Session 1)Protein
- 17A selenium-activated protein P catalyses a metabolic reaction that results in release of two ions. Expression of P is induced by lactose. In2020 (Session 2)Enzymes
- 18Height of a plant is positively determined by the amount of final product, K, of a metabolic pathway catalyzed by five enzymes and (as depic2020 (Session 2)Enzymes
- 19Proteins in biological systems are polypeptides that are exclusively made from Lamino acids. A peptide, , with L-amino acids of a particular2020 (Session 2)Protein
- 20Radioisotopes are frequently used in the study of cellular processes. A culture of Escherichia coli is grown at in a minimal medium containi2019 (Session 1)Nucleic Acid
- 21A scientist performed enzyme kinetics and obtained velocity as a function of substrate concentration and the values are given in the table b2019 (Session 1)Enzymes
- 22Trypsin is a digestive enzyme of serine protease group. It is considered an endopeptidase, i.e., the cleavage occurs within the polypeptide 2019 (Session 2)Enzymes
- 23The graph given below shows the Michaelis-Menten kinetics for two reactions (R1 and R2; represented as solid and dotted lines, respectively)2018Enzymes
- 24Consider the following statements regarding the Michaelis-Menten's constant . I : is indicative of the affinity of an enzyme for its substra2017Enzymes
- 25Formation of both peptide bonds and glycosidic bonds2015Carbohydrates
- 26A bacterial culture grown for 48 hrs in a medium containing radioactive sulphur would incorporate the radiolabel in the tetra-peptide:2015Protein
- 27A graph showing the effect of substrate concentration on enzyme activity is given below. The correct statement(s) is/are:2015Enzymes
- 28An experimental setup to study the effect of pH on the activity of the enzyme amylase is shown in the figure. The tubes A and B are made of 2014Enzymes
- 29What is the type of linkage between - and phosphates in nucleoside triphosphates?2011Nucleic Acid
Frequently Asked Questions
1.How many questions are available for Biomolecules (B)?
This chapter includes 29 questions compiled from NEST (NISER) papers spanning 2011 to 2024.
2.Are these questions only from NEST (NISER), or from other exams too?
These are NEST (NISER) questions specifically for the Biomolecules (B) chapter. Questions from other exams like JEE Main or NTA Abhyas (JEE Main) are organised separately under their respective exam sections.
3.Is Biomolecules (B) an important chapter for NEST (NISER) Biology?
Biomolecules (B) has appeared in 11 of the 16 years of NEST (NISER) papers covered here, with 29 questions across 16 papers - about 2 per paper it appears in.
4.What is the difficulty level of Biomolecules (B) questions in NEST (NISER)?
Of the 29 questions analysed for this chapter, 7% are easy, 79% moderate and 14% tough. 2024 had the most, with 7 questions.
5.Which topics in Biomolecules (B) should I focus on?
These topics have carried the most weightage in Biomolecules (B):
- Enzymes - 15 questions (52%)
- Protein - 8 questions (28%)
- Nucleic Acid - 4 questions (14%)
- Protein has produced the most tough questions (3).
6.Is Biomolecules (B)'s weightage in NEST (NISER) going up or down?
Across all 16 papers analysed, Biomolecules (B) has averaged about 1.8 questions per paper. In the last three years (2024–2026), that average has stayed about the same, at about 1.8 questions per paper. Use the year-wise numbers on this page for the exact trend before you decide how much time to give it.