Q2 / 68 · NEST (NISER) 2022 (Session 1) · Biology

A student working in a lab had three tubes with the following preparations:
(i) Freshly extracted nucleic acids dissolved in a buffer.
(ii) Chlorophyll extract from spinach leaf.
(iii) Red coloured anthocyanin from onion cells.

The student used a spectrophotometer to determine the wavelength of light at which each preparation would show maximum absorption (absorption maxima). She noted down these values in her notebook. However, when she came to the lab the next day she realised that she had not mentioned the name of the sample preparation against each value. The values she recorded were $480 \mathrm{~nm}, 260 \mathrm{~nm}$ and 642 nm . The respective values for the samples (i), (ii) and (iii) would most likely be:

  • A
    $480 \mathrm{~nm} ; 260 \mathrm{~nm}$ and 642 nm.
  • Correct answer
    B
    $260 \mathrm{~nm} ; 642 \mathrm{~nm}$ and 480 nm.
  • C
    $480 \mathrm{~nm} ; 642 \mathrm{~nm}$ and 260 nm.
  • D
    $260 \mathrm{~nm} ; 480 \mathrm{~nm}$ and 642 nm.

Hence, the correct answer is option: 'B'.

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