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Biology - Biotechnology - Principles and Processes

Practise 28 MHT-CET (PCB) previous year questions on Biotechnology - Principles and Processes, compiled from papers in 2026. Each question is pulled directly from the actual exam paper it appeared in, so you're practising in the exact style and difficulty MHT-CET (PCB) tests.

Biotechnology - Principles and Processes has been asked in 1 of the 1 years of MHT-CET (PCB) papers analysed (2026), with 28 questions in all. 64% of them were easy, 36% moderate and 0% tough. Most of its questions came from 2026, with 28 questions that year. In 2026 it had 28 questions across 11 papers, about 2.5 per paper.

The chapter's 4 topics are not weighted equally: Tools of Recombinant DNA Technology (16 questions, 57%), Separation and Isolation of DNA Fragments (10 questions, 36%), and Principles of Biotechnology (1 question, 4%) have been asked the most.

  1. 1During PCR cycle, the temperature range in the step of annealing is __________.26 Apr, 2026 (M)Separation and Isolation of DNA Fragments
  2. 2Originally, restriction endonucleases (REN) are named so because they __________26 Apr, 2026 (M)Conceptual Development of Genetic Engineering
  3. 3In biotechnology, the transfer of rDNA into the cloning host bacterium is assisted by __________26 Apr, 2026 (M)Tools of Recombinant DNA Technology
  4. 4Which one of the following pair of microbes shows significant role in genetic engineering experiments?25 Apr, 2026 (M)Tools of Recombinant DNA Technology
  5. 5Beginning with 20 fragments of DNA, after 10 cycles of PCR, how many copies of DNA will be produced?25 Apr, 2026 (M)Separation and Isolation of DNA Fragments
  6. 6The source of restriction endonuclease Alu I is ________ .25 Apr, 2026 (M)Tools of Recombinant DNA Technology
  7. 7Restriction enzymes are25 Apr, 2026 (M)Tools of Recombinant DNA Technology
  8. 8The technique of PCR was discovered in 1985 by _________.25 Apr, 2026 (E)Separation and Isolation of DNA Fragments
  9. 9The amplified DNA fragments can be separated by ___________.25 Apr, 2026 (E)Separation and Isolation of DNA Fragments
  10. 10Vehicle DNA is the common name given to which of the following?25 Apr, 2026 (E)Tools of Recombinant DNA Technology
  11. 11Which one of the following is NOT used as competent host in rDNA technology?25 Apr, 2026 (E)Tools of Recombinant DNA Technology
  12. 12'PAGE, SDS PAGE' are the methods of which of the following?24 Apr, 2026 (M)Separation and Isolation of DNA Fragments
  13. 13The enzyme Taq polymerase during PCR cycle is actually involved in which of the following steps?24 Apr, 2026 (M)Separation and Isolation of DNA Fragments
  14. 14The restriction endonuclease responsible for cleaving the given restriction site is ___________.24 Apr, 2026 (M)Tools of Recombinant DNA Technology
  15. 15In genetic engineering, the term chimeric DNA is applied to _________.24 Apr, 2026 (M)Principles of Biotechnology
  16. 16The device required for PCR is called thermal cycler. How many copies of the DNA segments are produced at the end of 9 cycles in an automati24 Apr, 2026 (E)Separation and Isolation of DNA Fragments
  17. 17Which of the following enzymes is used to insert the foreign DNA into the vector DNA?24 Apr, 2026 (E)Tools of Recombinant DNA Technology
  18. 18Which enzyme is used for inserting foreign DNA into the vector DNA in recombinant DNA technology?23 Apr, 2026 (M)Tools of Recombinant DNA Technology
  19. 19Which of the following characteristics, a good cloning vector must possess, to be efficiently used in rDNA technology? (A). It should have t23 Apr, 2026 (M)Tools of Recombinant DNA Technology
  20. 20By using the enzyme DNA ligase, foreign DNA is integrated into the vector. The combination of vector DNA and foreign DNA is called23 Apr, 2026 (E)Tools of Recombinant DNA Technology
  21. 21Rearrange the steps of gene cloning and choose the correct option: i) Isolation of DNA from donor organism ii) Isolation of a desired fragme23 Apr, 2026 (E)Separation and Isolation of DNA Fragments
  22. 22Which one of the following is NOT a technique used for isolation and characterization of macromolecules?22 Apr, 2026 (M)Separation and Isolation of DNA Fragments
  23. 23Which one of the following palindrome sequence is recognized by restriction enzyme EcoRI?22 Apr, 2026 (M)Tools of Recombinant DNA Technology
  24. 24Identify the correct statements regarding biotechnology. (A). Exonucleases cut the nucleotides at the ends of DNA strands. (B). The sequence22 Apr, 2026 (E)Tools of Recombinant DNA Technology
  25. 25Which of the following techniques are used for the transfer of foreign DNA into the host cell? (A). Microinjection (B). Shot gun (C). Ultras22 Apr, 2026 (E)Tools of Recombinant DNA Technology
  26. 26Given below are two statements: Statement (I): In rDNA technology, the transformation process generates a mixed population of transformed an21 Apr, 2026 (M)Tools of Recombinant DNA Technology
  27. 27Which of the following is INCORRECT in transformation of rDNA into suitable competent host?21 Apr, 2026 (E)Tools of Recombinant DNA Technology
  28. 28Which of the following techniques can be used to transfer foreign DNA directly into the cell or protoplasm without the use of plasmids? (i) 21 Apr, 2026 (E)Separation and Isolation of DNA Fragments

Frequently Asked Questions

1.How many questions are available for Biotechnology - Principles and Processes?

This chapter includes 28 questions compiled from MHT-CET (PCB) papers in 2026.

2.Are these questions only from MHT-CET (PCB), or from other exams too?

These are MHT-CET (PCB) questions specifically for the Biotechnology - Principles and Processes chapter. Questions from other exams like NEET or AIIMS are organised separately under their respective exam sections.

3.Is Biotechnology - Principles and Processes an important chapter for MHT-CET (PCB) Biology?

Biotechnology - Principles and Processes has appeared in 1 of the 1 year of MHT-CET (PCB) papers covered here, with 28 questions across 11 papers - about 3 per paper it appears in.

4.What is the difficulty level of Biotechnology - Principles and Processes questions in MHT-CET (PCB)?

Of the 28 questions analysed for this chapter, 64% are easy, 36% moderate and 0% tough. 2026 had the most, with 28 questions.

5.Which topics in Biotechnology - Principles and Processes should I focus on?

These topics have carried the most weightage in Biotechnology - Principles and Processes:

  • Tools of Recombinant DNA Technology - 16 questions (57%)
  • Separation and Isolation of DNA Fragments - 10 questions (36%)
  • Principles of Biotechnology - 1 question (4%)
6.Is Biotechnology - Principles and Processes's weightage in MHT-CET (PCB) going up or down?

Across all 11 papers analysed, Biotechnology - Principles and Processes has averaged about 2.5 questions per paper. In 2026, that average has stayed about the same, at about 2.5 questions per paper. Use the year-wise numbers on this page for the exact trend before you decide how much time to give it.