Rearrange the steps of gene cloning and choose the correct option:
i) Isolation of DNA from donor organism
ii) Isolation of a desired fragment
iii) Transferring the recombinant to the host cell
iv) Fragmentation of DNA by restriction endonuclease
v) Ligation of fragment to vector
vi) Culturing host cell in medium
Choose the correct answer from the options given below:

The correct sequence of steps involved in gene cloning (recombinant DNA technology) is as follows:

1. Isolation of DNA from the donor organism (i).

2. Fragmentation of DNA by restriction endonucleases (iv).

3. Isolation of the desired DNA fragment (ii).

4. Ligation of the DNA fragment into a vector to form recombinant DNA (v).

5. Transferring the recombinant DNA into the host cell (iii).

6. Culturing the host cells in a medium at a large scale to obtain the desired product (vi).

Therefore, the correct sequence is i $\rightarrow$ iv $\rightarrow$ ii $\rightarrow$ v $\rightarrow$ iii $\rightarrow$ vi.

Answer: i--iv---ii--v---iii---vi