Q24 / 200 · MHT CET - Medical 2026 (25 April Shift 2) · Biology

The amplified DNA fragments can be separated by ___________.

  • A
    autoradiography
  • Correct answer
    B
    gel electrophoresis
  • C
    x-ray crystallography
  • D
    ultra centrifugation

Solution

Amplified DNA fragments are separated according to their size through a technique called gel electrophoresis.

DNA fragments are negatively charged molecules, so they can be separated by forcing them to move towards the anode under an electric field through a matrix or gel. The most commonly used matrix is agarose, a natural polymer extracted from seaweeds.

The DNA fragments separate (resolve) according to their size through the sieving effect provided by the agarose gel. Hence, the smaller the fragment size, the farther it moves.

Answer: gel electrophoresis

Hence, the correct answer is option: 'B'.

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