Which enzyme is used for inserting foreign DNA into the vector DNA in recombinant DNA technology?

In recombinant DNA technology, the insertion of foreign DNA into a vector DNA requires the joining of their ends. This process is facilitated by the enzyme DNA ligase, which forms phosphodiester bonds between the adjacent nucleotides of the foreign DNA and the vector DNA, effectively sealing the nicks and creating a continuous recombinant DNA molecule.

DNA polymerase is involved in synthesizing new DNA strands.

DNA gyrase (a type of topoisomerase) relieves torsional strain during DNA replication.

DNA helicase unwinds the DNA double helix.

Answer: DNA ligase