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NEST (NISER) 2025

Browse the complete Biology, Chemistry, Mathematics, and Physics question set from the NEST (NISER) 2025 paper, question by question. Each question shows a quick preview so you can jump straight to the topic or subject you want to revise. Click through to attempt the full question in practice mode, or head back to the paper-wise list to download the entire paper as a PDF.

This paper has 80 questions in total - 5 easy (6%), 62 moderate (78%) and 13 tough (16%). That's a fairly typical mix for this exam. By subject: Biology 5% easy, 75% moderate, 20% tough; Chemistry 10% easy, 75% moderate, 15% tough; Mathematics 0% easy, 70% moderate, 30% tough; Physics 10% easy, 90% moderate, 0% tough.

Biology questions were spread across 12 chapters, with Evolution (3), Cell Cycle and Cell Division (2), and Molecular Basis of Inheritance (2) carrying the most weight. Chemistry questions were spread across 16 chapters, with Biomolecules (3), Chemical Bonding and Molecular Structure (2), and Coordination Compounds (2) carrying the most weight. Mathematics questions were spread across 13 chapters, with Definite Integration (3), Basic of Mathematics (2), and Functions (2) carrying the most weight. Physics questions were spread across 16 chapters, with Ray Optics (2), Thermodynamics (2), and Waves and Sound (2) carrying the most weight.

Biology

  1. 1Change of amino acids in a protein led to formation of a salt bridge, without affecting the overall fold of the protein. Such a change will 2025Protein
  2. 2Consider the following statements regarding steps involved in the light reaction of photosynthesis. (I) Water is oxidized and protons are re2025Mechanism of Photosynthesis
  3. 3Malaria, caused by Plasmodium, is transmitted by Anopheles mosquito. Plasmodium transforms into a virulent form , and is stored in the saliv2025Common Health Disease
  4. 4The oxygen dissociation curve (as percentage saturation versus of blood) of haemoglobin for a healthy adult individual is shown in the graph2025Human Respiratory System
  5. 5When a human somatic cell undergoes mitotic division, the ploidy level in metaphase and telophase, respectively, would be:2025Cell Cycle Involves Two Stages
  6. 6In an enzymatic reaction, the initial velocity at a substrate concentration of 0.03 was . The substrate concentration required to reach half2025Enzymes
  7. 7In an experiment, 250 bacterial cells were inoculated into a flask containing fresh growth medium. After a period of acclimatization, the gr2025Introduction
  8. 8In the eukaryotic cell cycle shown in the figure, the black solid bars (1, and 3) represent important checkpoints that determine whether the2025Cell Cycle Involves Two Stages
  9. 9Shotgun method was used for sequencing a stretch of DNA wherein the molecule was broken into small fragments and each fragment was sequenced2025DNA Fingerprinting
  10. 10In the lac-operon system, lactose acts as an inducer that binds to the repressor protein attached to the operator region (lacO) and allows t2025Regulation of Gene Expression
  11. 11Two plants of the same type were potted in separate pots and . After a few days, it was observed that the leaves of the plant in pot were co2025Plant Water Relations
  12. 12The pattern formation of certain tissues ( and ) in a thickening dicot stem is depicted in the figure. If a transverse section of such a ste2025Permanent Tissues
  13. 13Four pedigrees are shown, where square represents male and circle represents female. Affected individuals are represented as filled squares 2025Genetic Disorders
  14. 14When a true-breeding purple-fruit-bearing brinjal plant is crossed with a true-breeding white-fruit-bearing brinjal plant, all the F1 plants2025Incomplete Dominance
  15. 15Experiments carried out by the ethologist Nikolaas Tinbergen supported the hypothesis that 'a wasp learns the location of her nest by visual2025Response to Abiotic Factors
  16. 16The table depicts list of characters (I-V) in five species (M-Q) where 1 represents presence and 0 represents absence of the character. Spec2025Evidences of Evolution
  17. 17Studies have shown that considerable genetic variation exist in the number of abdominal bristles found in fruit fly populations. An experime2025Evidences of Evolution
  18. 18The process of opening and closing of stomata (called stomatal movement) is controlled by turgor pressure changes in the guard cells. A list2025Transpiration
  19. 19A researcher wants to study the effect of habitat fragmentation on bird species richness in a tropical rainforest. The species richness of t2025On the Basis Development
  20. 20A particular herbivore species was being studied. There are two populations of this species, one found in Sri Lanka and the other in Indian 2025Modern Theory of Evolution

Chemistry

  1. 1Maltose, a disaccharide, contains glycosidic linkage between2025Disaccharides and Polysaccharides
  2. 2Consider the following component The IUPAC name of the compound is2025IUPAC Nomenclature
  3. 3According to the VSEPR theory, the shapes of , and , respectively, are2025Hybridisation and VSEPR Theory
  4. 4If the density of a liquid is , then the mass of 1.2 mL of the liquid in correct significant figures is2025Concentration terms
  5. 5The oxidation number and valence number of Ga in having a metal-metal bond, respectively, are2025Properties of D-block elements
  6. 6Cyclopentene is transformed into 1,2,3-tribromocyclopentane in two steps. The correct order of reagents required to carry out this transform2025Reactions of alkenes
  7. 7The compound on reaction with KCN gives which on catalytic hydrogenation with produces . Compound on reaction with gives n-propylisocyanide.2025Preparation and Properties of Isocyanides
  8. 8Consider the following compound. The major product in an aromatic electrophilic substitution reaction ( electrophile) on the given compound 2025Reactions of aromatic compounds
  9. 9Consider the following reaction: The compound P is2025Method of preparation for Ketones
  10. 10Isopropyl benzene when oxidized in the presence of air followed by treatment with dil. HCl produces compounds and turns a blue litmus paper 2025Reactions of Alcohols
  11. 11A first-row transition metal dipositive ion forms an octahedral aqua complex , which is paramagnetic. The addition of excess ammonia to give2025Werner's theory and Valence Bond Theory
  12. 12The correct sequence of the given manganese oxides with respect to melting point is2025Properties of D-block elements
  13. 13The metal ion that does not form a stable metal carbonyl complex is2025Crystal Field Theory
  14. 14Consider two radioactive substances and that decay following a first order kinetics. It takes nine years for for decay of of its initial amo2025Integrated rate laws
  15. 15Consider the reaction performed in a 1 L container. The equilibrium constant is equal to 0.5 . If the reaction begins with 1 mole of moles o2025Law of Mass Action, Equilibrium Constant (Kc and Kp) and its Application
  16. 16For a photoelectric setup, the relationship between the maximum velocity of the photoelectrons and the incident radiation frequency ( being 2025Dual Behaviour of Matter and Heisenberg Uncertainty Principle
  17. 17Consider a hypothetical order of molecular orbitals (MO) as shown below. Assuming this MO ordering is valid for all the given diatomic molec2025Molecular Orbital Theory
  18. 18Let the vapour pressure of a liquid be and at two different temperatures and , respectively. They are related by an equation given by where,2025Vapour pressure and raoult's law
  19. 19The Gibbs free energy G for a liquid is considered as a function of temperature and pressure is plotted either as a function of temperature 2025Third law of thermodynamics
  20. 20Consider the electrochemical cell with two metal/metal ion electrodes, represented as , where, M and N represent two different metals. The p2025Cells and Electrode Potential, Nernst Equation

Mathematics

  1. 1Let , for . Consider the following system of two equations in the variables and : This system does not have a unique solution if and only if2025Determinants
  2. 2Let be the set of all rational numbers such that if with , then the two solutions of the quadratic equation are equal. Then the number of el2025Relation between Roots and Coefficients
  3. 3The value of the integral is2025Definite Integration by Substitution
  4. 4For , define . Then for any arbitrary pair of real numbers ,2025Functions
  5. 5Let and be two lines in the 3-dimensional space. The line is defined by the equations and the line is defined by the equations where is a no2025Line in Space
  6. 6A circle with centre at and radius touches another circle with centre at and radius internally as shown in the figure. If and a diameter of 2025Tangent to circle
  7. 7Let be defined by . Then is2025Differentiability
  8. 8Let be a complex number such that but . Then is equal to2025De Moivres Theorem
  9. 9Let and be three 3 -digit numbers chosen randomly. Then the probability that is divisible by number 3 is2025Classical Definition of Probability
  10. 10Let , where are nonzero integers. Suppose that . Then, all the roots of are integers if and only if2025N degree equation
  11. 11If and are two real numbers such that then the value of is2025Algebraic and Rational Limits
  12. 12Let be a continuous function such that Then the value of is2025Differential Equations
  13. 13The value of is2025Definite Integration by Parts
  14. 14An ellipse has the points and as foci. If the ellipse touches the -axis then its eccentricity is2025Ellipse
  15. 15Let and be two students who have appeared NEST. Given that i. the probability that X qualifies NEST is ; ii. the probability that X qualifie2025Conditional Probability and Multiplication Theorem
  16. 16Let and be two sets. Let and be subsets of such that and for . Let be a function. For each , let be defined by for . Then2025Functions
  17. 17Let be the equivalence relation on defined by for if is a rational number. Then2025Logarithm
  18. 18Let be the set of integers such that and can be written as , with . Then the number of elements in is2025Inequalities
  19. 19The limit2025Definite Integration
  20. 20Let and be points in . Then the distance between the lines AB and OC is2025Line in Space

Physics

  1. 1In 2024 Olympics two runners, Noah Lyles and Kishane Thompson, finished the 100.00 m race in 9.784 s and 9.789 s respectively. Assuming that2025Rest and Motion
  2. 2Three positive and two negative charges each of magnitude are placed at five of the six vertices of a regular hexagon of side . The work don2025Electric Potential and Potential Energy
  3. 3Consider a metal cylinder of heat capacity , thermal conductivity , mass and radius . The combination that will have the dimension of time i2025Dimensions
  4. 4If all the Helium-4 nuclei, each of mass 4.0026034 u , in the core of a star is converted to Carbon-12 nuclei, then the energy released in M2025Nuclear energy
  5. 5The figure shows a ray of light going from a medium of refractive index to a medium of refractive index such that . Select the correct optio2025Refraction of light from plane surface
  6. 6A simple pendulum, suspended from the roof of a lift, is set to small oscillations. The lift starts moving downwards such that the distance 2025Applications of SHM
  7. 7A periodic dimming of light coming from a distant star is observed. This is attributed to the eclipses caused by the presence of a planet or2025Kepler laws
  8. 8A bead is constrained to move on a frictionless wire, placed in the plane and under the influence of gravity. The shape of this wire is desc2025Energy
  9. 9The engine in a sports car provides constant power to all the four wheels over a range 0 to 120 kilometers per hour (kmph). The car accelera2025Power
  10. 10Consider the following circuit with capacitors and having equal capacitance of . Two switches and are closed simultaneosuly at . After a lon2025Capacitance
  11. 11The transverse electric field of an electromagnetic wave in free space is expressed as , where is the propagation vector and is a constant. 2025Electromagnetic Waves and Maxwell's equations
  12. 12A square loop of side length , carrying current , is placed in the plane as shown in the figure. The magnetic field in the region has the fo2025Force and Torque on Current Carrying Conductor
  13. 13A qualitative phase diagram of a substance is shown in the figure below. Choose the correct option from the following statements.2025Thermodynamic Systems
  14. 14An ideal monoatomic gas, initially at pressure , is kept in a spherical container of volume . The container is adiabatically compressed to h2025Thermodynamic Processes
  15. 15The Parker solar probe, orbiting the Sun, experiences solar radiation flux of 650 . The heat shield (area , emissivity ) surrounding the pro2025Radiation
  16. 16A p-n junction diode in a circuit is in reverse bias but not under breakdown condition. As compared to the p-n junction diode in a forward b2025Diodes
  17. 17Monochromatic light from a discharge tube containing dilute gas of hydrogen atoms falls on platinum metal which has a work function of 5.65 2025Photoelectric Effect
  18. 18A tube of length 0.5 m open at both ends has a small hole in the middle. Air is blown through this hole. If the speed of sound in air is , t2025Vibration of String
  19. 19Four long glass plates with refractive indices are stacked in parallel as shown in the figure. A light ray, traveling in the first plate, is2025Critical angle and total internal reflection
  20. 20The magnitude of an earthquake is related to the energy it releases by a logarithmic scale, , where and are positive constants. An earthquak2025Properties of Sound Waves

Frequently Asked Questions

1.How many questions are in the NEST (NISER) 2025 paper?

This paper has 80 questions in total - 20 each from Biology, Chemistry, Mathematics, and Physics.

2.Can I practise questions from just one subject?

Yes. Use the Biology, Chemistry, Mathematics, and Physics tabs at the top of the page to jump directly to that subject's question set within this paper.

3.How is the NEST (NISER) 2025 paper structured?

The NEST (NISER) 2025 paper on MARKS has 80 questions, organised by subject:

  • Biology: 20 questions - 20 single-correct.
  • Chemistry: 20 questions - 20 single-correct.
  • Mathematics: 20 questions - 20 single-correct.
  • Physics: 20 questions - 20 single-correct.
4.How difficult is the NEST (NISER) 2025 paper?

Each question on MARKS carries a difficulty rating. Of the 80 rated questions in this paper, 5 (6%) are easy, 62 (78%) are medium and 13 (16%) are hard. Subject wise:

  • Biology: 1 easy, 15 medium, 4 hard
  • Chemistry: 2 easy, 15 medium, 3 hard
  • Mathematics: 0 easy, 14 medium, 6 hard
  • Physics: 2 easy, 18 medium, 0 hard
5.Which chapters were asked the most in the NEST (NISER) 2025 paper?

The chapters with the most questions in each subject were:

  • Biology: Evolution (3), Biomolecules (2), and Cell Cycle and Cell Division (2).
  • Chemistry: Chemical Bonding and Molecular Structure (2), Coordination Compounds (2), and d and f Block Elements (2).
  • Mathematics: Definite Integration (3), Basic of Mathematics (2), and Functions (2).
  • Physics: Ray Optics (2), Thermodynamics (2), and Waves and Sound (2).
6.Where can I find the solutions for the NEST (NISER) 2025 paper?

Open any question on this page. Its page shows the correct answer and, where one is available, the worked solution. A downloadable PDF of the whole paper is coming soon.

7.How should I use the NEST (NISER) 2025 paper to check my preparation?

Set a timer, attempt the 80 questions as you would in the exam, and only then open the answers. Note which subjects and chapters cost you the most marks, and revise those first using the chapter-wise practice for NEST (NISER). Repeating this with other papers shows whether the same weak spots keep coming back.

8.Will the NEST (NISER) 2025 paper help me prepare for NEST (NISER) 2027?

Yes. Every past paper shows the question styles and topics NEST (NISER) has asked before, and a solved paper gives you a realistic benchmark for speed and accuracy. Use it along with the other papers to spot the chapters that keep returning.