Q193 / 200 · NEET 2012 (Screening) · Zoology

Which one is a true statement regarding DNA polymerase used in PCR?

  • A
    It is used to ligate introduced DNA in recipient cells
  • B
    It serves as a selectable marker
  • C
    It is isolated from a virus
  • Correct answer
    D
    It remains active at high temperature

Solution

Polymerase Chain Reaction (PCR) is used to amplify a DNA segment or to synthesize in vitro the multiple copies of gene (or DNA) of interest, using two sets of primers and the enzyme DNA polymerase. This enzyme is isolated from a bacterium Thermus aquaticus and it remains active during the high temperature but high temperature induced denaturation of double stranded DNA.

Hence, the correct answer is option: 'D'.

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