Q175 / 180 · AIIMS 2004 · Zoology

What is the firststep in the Southern Blottechnique?

  • A
    Denaturation of DNA on the gel for hybridization with specific probe
  • B
    Production of a group of genetically identical cells
  • C
    Digestion of DNA by restriction enzyme
  • Correct answer
    D
    Isolation of DNA from a nucleated cell such as the one from the scene of crime

Solution

Southern blotting was named after Edward M. Southern who developed this procedure in Edinburgh University in the 1970's. Southern blotting is designed to locate a particular sequence of DNA within a complex mixture. The amount of DNA needed for this technique is dependent on the size and specific activity of the probe. Before, starting the procedure, the desired DNA should be isolated from a specific nucleated cell.

Hence, the correct answer is option: 'D'.

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