Q118 / 120 · NEET 2012 (Mains) · Zoology

The figure below shows three steps (I, II, III) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? Figure

  • A
    B-Denaturation at a temperature of about $98^{\circ} \mathrm{C}$ separating the two DNA strands
  • B
    A-Denaturation at a temperature of about $50^{\circ} \mathrm{C}$
  • Correct answer
    C
    C-Extension in the presence of heat stable DNA polymerase
  • D
    A-Annealing with two sets of primers

Solution

There are three steps in polymerase chain reaction namely. (i) Denaturation-at $94^{\circ} \mathrm{C}$ for DNA strand separation. (ii) Annealing-binding of primer. (iii) Extension in presence of DNA polymerase which is heat stable.

Hence, the correct answer is option: 'C'.

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