Q176 / 180 · NEET 2013 (All India) · Zoology
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
- ANon-recombinant bacteria containing $\beta$-galactosidase
- BInsertional inactivation of $\alpha$-galactosidase in non-recombinant bacteria
- Correct answerCInsertional inactivation of $\alpha$-galactosidase in recombinant bacteria
- DInactivation of glycosidase enzyme in recombinant bacteria
Solution
The colonies of recombinant bacteria appear white in contrast to blue calories of non-recombinant bacteria because of insertional inactivation of alpha galactosidase in recombinant bacteria. Alpha galactosidase is a glycoside hydrolase enzyme that hydrolyse the terminal alpha galactosyl moieties from glycolipids and glycoprotein. It is encoded by the GLA gene. $\beta$-galactosidase is an exaglycosidase, which hydrolyzes the $\beta$-glycosidic bond formed between a galactose and its organic rhoiety.
Hence, the correct answer is option: 'C'.