Q185 / 200 · NEET 2021 · Zoology

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because:

  • A
    Mutated gene partially appears on a photographic film
  • B
    Mutated gene completely and clearly appears on a photographic film
  • Correct answer
    C
    Mutated gene does not appear on a photographic film as the probe has no complementarity with it
  • D
    Mutated gene does not appear on photographic film as the probe has complementarity with it

Solution

During cancer detection, for the presence of the mutated gene by using the autoradiographic technique, the complementary gene does not bind to the mutated gene, and hence in the photographic film, the gene is not observed, because the probe will not have the complementarity with the mutated gene.

Hence, the correct answer is option: 'C'.

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