Q174 / 180 · NEET 2020 (Phase 2) · Zoology

In Recombinant DNA technology antibiotics are used:

*Note: There was ambiguity in options in the original paper, hence the options are changed.

  • A
    To keep medium bacteria-free
  • B
    To detect alien DNA
  • C
    As selectable markers
  • Correct answer
    D
    Both (B) and (C)

Solution

Selectable marker: Helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E.coli. 

During the screening, it helps in differentiating the cells that have alien DNA or gene of interest.

Hence, the correct answer is option: 'D'.

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