Q178 / 180 · NEET 2019 (All India) · Zoology

Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.

A
The enzyme cuts DNA molecules at identified position within the DNA.
Correct answer
B
The enzyme binds DNA at specific sites and cuts only one of the two strands.
C
The enzyme cuts the sugar-phosphate backbone at specific site on each strand.
D
The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.

Solution

DNA consists of two complementary strands of nucleotides that spiral around each other in a double helix. Restriction enzymes cut through both nucleotide strands, breaking the DNA into fragments. These are the endonucleases that are used in RDT or genetic engineering techniques.

Hence, the correct answer is option: 'B'.

Relevant Questions

In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to selec2025Tools of Recombinant DNA Technology
Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E2025Tools of Recombinant DNA Technology
The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basi2025Tools of Recombinant DNA Technology
What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism? A. The piece of DNA would be 2024Tools of Recombinant DNA Technology
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:2024Tools of Recombinant DNA Technology
The "Ti plasmid" of Agrobacterium tumefaciens stands for2024Tools of Recombinant DNA Technology