Q10 / 67 · NEST (NISER) 2021 (Session 1) · Biology
A linear double-stranded DNA was completely digested using either one or two restriction endonucleases ( $\mathrm{C} f r 10 \mathrm{I}$ and BanII). The sequence of one of the strands is provided. The restriction site for Cfr10I is RCCGGY and for BanII is GRGCYC ( $\mathbf{R}$ is A or G and $\mathbf{Y}$ is T or C ).
5'TGGAGAGACCGGTAGCAGAGAGCTCCATTCCGGGCGCGCCGGCACCCTTAGGA3'
Based on this information, the correct option is
5'TGGAGAGACCGGTAGCAGAGAGCTCCATTCCGGGCGCGCCGGCACCCTTAGGA3'
Based on this information, the correct option is
- ADigestion with both C fri0I and BanII will generate three fragments.
- Correct answerBDigestion with both Cfr10I and BanII will generate four fragments.
- CDigestion with BanII will generate one fragment.
- DDigestion with Cfr10I will generate two fragments.
Hence, the correct answer is option: 'B'.