Q4 / 68 · NEST (NISER) 2023 (Session 1) · Biology

A 1000 base pair double-stranded DNA (B form) has a melting temperature $\left(T_m\right)$ of $58^{\circ} \mathrm{C}$. If a duplex RNA (A form) of the same length and sequence is constructed, then the $T_m$ of this new RNA duplex with respect to the DNA (B form) would be

  • Correct answer
    A
    higher due to greater stability of A form of RNA duplex.
  • B
    lower due to lower stability of A form of RNA duplex.
  • C
    lower because of unfavourable enthalpy of formation of RNA duplex.
  • D
    identical, as the number of hydrogen bonds remain the same.

Hence, the correct answer is option: 'A'.

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