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Biology - Biotechnology - Principles and Processes

Practise 11 IAT (IISER) previous year questions on Biotechnology - Principles and Processes, compiled from papers spanning 2017 to 2026. Each question is pulled directly from the actual exam paper it appeared in, so you're practising in the exact style and difficulty IAT (IISER) tests.

Biotechnology - Principles and Processes has been asked in 8 of the 9 years of IAT (IISER) papers analysed (2017–2026), with 11 questions in all. 18% of them were easy, 64% moderate and 18% tough. Most of its questions came from 2024, with 2 questions that year. In the last three years (2023–2026) it had 4 questions across 3 papers, about 1.3 per paper.

The chapter's 2 topics are not weighted equally: Tools of Recombinant DNA Technology (8 questions, 73%) and Separation and Isolation of DNA Fragments (3 questions, 27%) have been asked the most. Tools of Recombinant DNA Technology has also led over the last five years, with 5 questions. Tools of Recombinant DNA Technology has produced the most tough questions (2).

  1. 1The pBR322 cloning vector has genes coding for tetracycline and ampicillin resistance. A foreign DNA to be cloned is inserted into the tetra2026Tools of Recombinant DNA Technology
  2. 2The given picture was obtained from an agarose gel electrophoresis of a plasmid after digestion with restriction enzymes either or both X an2025Tools of Recombinant DNA Technology
  3. 3Which of the following plasmid vectors can be used for cloning of a gene, with restriction enzymes BamHI and EcoRI, and ampicillin-containin2024Tools of Recombinant DNA Technology
  4. 4Polymerase chain reaction (PCR) is used to amplify a gene of interest (GOI). If, after 30 cycles of PCR, 1 billion copies of GOI are produce2024Separation and Isolation of DNA Fragments
  5. 5A 1000 base-pair DNA fragment was cloned between Hind III and EcoR I sites of the plasmid vector (pIAT) of size 3500 base-pair. The cloned f2023Tools of Recombinant DNA Technology
  6. 6As opposed to DNA replication within the cell, discontinuous synthesis of DNA does NOT occur in a polymerase chain reaction (PCR). Why?2022Tools of Recombinant DNA Technology
  7. 7The map of a 4000 base pair (bp) plasmid DNA marking the locations of different restriction enzyme cut sites is shown in the figure below. T2021Tools of Recombinant DNA Technology
  8. 8Which among the following correctly represents the sequence of events for a normal polymerase chain reaction?2019Separation and Isolation of DNA Fragments
  9. 9DNA fragments of 100 base pairs (bp), 300 bp and 500 bp were separated by agarose gel electrophoresis. Pick the correct arrangement of the f2018Separation and Isolation of DNA Fragments
  10. 10A gene is inserted into the PvuII site of the cloning vector (given below) and transformed into E. coli cells. Which one of the following st2018Tools of Recombinant DNA Technology
  11. 11Provided below are recognition sequences for restriction enzymes 1 (RE1), 2 (RE2) and 3 (RE3). Arrows indicate the positions where the enzym2017Tools of Recombinant DNA Technology

Frequently Asked Questions

1.How many questions are available for Biotechnology - Principles and Processes?

This chapter includes 11 questions compiled from IAT (IISER) papers spanning 2017 to 2026.

2.Are these questions only from IAT (IISER), or from other exams too?

These are IAT (IISER) questions specifically for the Biotechnology - Principles and Processes chapter. Questions from other exams like JEE Main or NTA Abhyas (JEE Main) are organised separately under their respective exam sections.

3.Is Biotechnology - Principles and Processes an important chapter for IAT (IISER) Biology?

Biotechnology - Principles and Processes has appeared in 9 of the 10 years of IAT (IISER) papers covered here, with 11 questions across 9 papers - about one per paper it appears in.

4.What is the difficulty level of Biotechnology - Principles and Processes questions in IAT (IISER)?

Of the 11 questions analysed for this chapter, 18% are easy, 64% moderate and 18% tough. 2024 had the most, with 2 questions.

5.Which topics in Biotechnology - Principles and Processes should I focus on?

These topics have carried the most weightage in Biotechnology - Principles and Processes:

  • Tools of Recombinant DNA Technology - 8 questions (73%)
  • Separation and Isolation of DNA Fragments - 3 questions (27%)
  • Tools of Recombinant DNA Technology has produced the most tough questions (2).
6.Is Biotechnology - Principles and Processes's weightage in IAT (IISER) going up or down?

Across all 9 papers analysed, Biotechnology - Principles and Processes has averaged about 1.2 questions per paper. In the last three years (2023–2026), that average has stayed about the same, at about 1.3 questions per paper. Use the year-wise numbers on this page for the exact trend before you decide how much time to give it.