Q44 / 180 · NEET 2026 (Re-NEET) · Botany

Match List-I with List-II.
List-IList-II
A. TransformationI. Restriction enzyme
B. Cloning siteII. Transfer DNA to host bacteria
C. SelectionIII. Replication
D. OriIV. Antibiotic
Choose the correct answer from the options given below :

  • A
    A-IV, B-I, C-III, D-II
  • Correct answer
    B
    A-II, B-I, C-IV, D-III
  • C
    A-I, B-II, C-IV, D-III
  • D
    A-III, B-IV, C-II, D-I

Solution

Transformation is a procedure through which a piece of DNA is introduced into a host bacterium. Thus, A matches with II.

Cloning sites are the recognition sites for restriction enzymes where the alien DNA is linked to the vector. Thus, B matches with I.

Selection of transformants from non-transformants is facilitated by selectable markers, which are typically genes encoding resistance to specific antibiotics. Thus, C matches with IV.

Ori (origin of replication) is the specific DNA sequence responsible for initiating replication. Thus, D matches with III.

The correct matching is A-II, B-I, C-IV, D-III.

Answer: A-II, B-I, C-IV, D-III

Hence, the correct answer is option: 'B'.

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