In meiosis, crossing over and exchange of genetic material between homologous chromosomes are catalyzed by the enzyme.

During Meiosis-I, the stage Prophase Ioccurs in which various substages are present. Of these substages, the most important is Pachytene stage in which exchange of chromosomal segments take place between non-sister chromatids of homologous chromosome, with the help of enzyme recombinase.