Q74 / 200 · MHT CET - Medical 2026 (24 April Shift 1) · Biology
Given below are two statements.
Statement I - There is no need of agitation in callus culture however it is necessary in suspension culture.
Statement II - During tissue culture techniques, the temperature of the lab is maintained between 18 to 20°C.
In the light of above statements, select the correct option given below:
Statement I - There is no need of agitation in callus culture however it is necessary in suspension culture.
Statement II - During tissue culture techniques, the temperature of the lab is maintained between 18 to 20°C.
In the light of above statements, select the correct option given below:
- ABoth statement I and statement II are correct.
- BBoth statement I and statement II are incorrect.
- Correct answerCStatement I is correct but statement II is incorrect.
- DStatement I is incorrect but statement II is correct.
Solution
Statement I is correct because callus culture is typically grown on a solid agar medium where agitation is not required. In contrast, suspension culture involves growing cells in a liquid medium, which requires continuous agitation (usually on a rotary shaker) to ensure proper aeration, nutrient mixing, and to prevent the aggregation of cells.
Statement II is incorrect because the standard temperature maintained in a plant tissue culture laboratory is generally around $24^{\circ}\text{C}$ to $28^{\circ}\text{C}$ (often specified as $25 \pm 2^{\circ}\text{C}$), not $18$ to $20^{\circ}\text{C}$.
Therefore, Statement I is correct but Statement II is incorrect.
Answer: Statement I is correct but statement II is incorrect.
Statement II is incorrect because the standard temperature maintained in a plant tissue culture laboratory is generally around $24^{\circ}\text{C}$ to $28^{\circ}\text{C}$ (often specified as $25 \pm 2^{\circ}\text{C}$), not $18$ to $20^{\circ}\text{C}$.
Therefore, Statement I is correct but Statement II is incorrect.
Answer: Statement I is correct but statement II is incorrect.
Hence, the correct answer is option: 'C'.