Q74 / 200 · MHT CET - Medical 2026 (24 April Shift 1) · Biology

Given below are two statements.
Statement I - There is no need of agitation in callus culture however it is necessary in suspension culture.
Statement II - During tissue culture techniques, the temperature of the lab is maintained between 18 to 20°C.
In the light of above statements, select the correct option given below:

  • A
    Both statement I and statement II are correct.
  • B
    Both statement I and statement II are incorrect.
  • Correct answer
    C
    Statement I is correct but statement II is incorrect.
  • D
    Statement I is incorrect but statement II is correct.

Solution

Statement I is correct because callus culture is typically grown on a solid agar medium where agitation is not required. In contrast, suspension culture involves growing cells in a liquid medium, which requires continuous agitation (usually on a rotary shaker) to ensure proper aeration, nutrient mixing, and to prevent the aggregation of cells.

Statement II is incorrect because the standard temperature maintained in a plant tissue culture laboratory is generally around $24^{\circ}\text{C}$ to $28^{\circ}\text{C}$ (often specified as $25 \pm 2^{\circ}\text{C}$), not $18$ to $20^{\circ}\text{C}$.

Therefore, Statement I is correct but Statement II is incorrect.

Answer: Statement I is correct but statement II is incorrect.

Hence, the correct answer is option: 'C'.

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