A transgenic crop plant $(2 \mathrm{n})$ was generated by transforming it with a kanamycinresistant gene ' $\mathbf{P}$ ' that integrates in its nuclear genome. Positive transformants were screened using this antibiotic as a selective agent. Southern blot analysis revealed a single copy insertion. Which of the following statement(s) is/are correct?

• A

  $P /-$ transgenic line can have stronger expression level of gene 'P' than the $\mathrm{P} / \mathrm{P}$ transgenic line.
• Correct answer
  B

  If the transformants are selfed, the offspring will have either $\mathrm{P} / \mathrm{P}, \mathrm{P} /-$ or -/- genotypes.
• Correct answer
  C

  The Southern analysis band pattern for $\mathrm{P} / \mathrm{P}$ and $\mathrm{P} /-$ will not be the same.
• Correct answer
  D

  $\mathrm{P} / \mathrm{P}, \mathrm{P} /-$ genotype plants should survive the selection.

Hence, the correct answer is options: 'B', 'C', 'D'.